Office of Research, UC Riverside
Office of Campus Veterinarian

Guidelines for Anesthesia and Analgesia

(Material adapted from the University of Iowa Animal Care Unit and Stanford University)

Anesthesia and Analgesia of Mice:
Anesthesia and Analgesia of Rats:
Anesthesia and Analgesia of Fish and Amphibians:

 

Anesthesia and Analgesia of Mice

Parenteral Anesthesia:

Dissociative Anesthetics

  • Ketamine, 80-100 mg/kg; + Xylazine, 10-20 mg/kg; IP
  • Ketamine, 70-100 mg/kg + Xylazine, 10-20mg/kg + Acepromazine, 2-3 mg/kg; IP

Barbiturates

  • Pentobarbital, 40-50 mg/kg; IP *narrow margin of safety.

Inhalant Anesthetic

  • Isoflurane 1-3% (up to 5% for induction)

Analgesics:

  • Ketoprofen, 2-5 mg/kg, every 8-12 hour; SQ
  • Buprenorphine, 0.05-0.1 mg/kg, q 6-12 hr.; SQ
  • Butorphanol, 1-5 mg/kg, q 4 -8hr, SQ
  • Carprofen 5 mg/kg, q 12-24 hr;SQ
  • Flunixin 2.5 mg/kg, q 12-24 hr;SQ
  • Meloxicam 5-10 mg/kg, q 12-24 hr;SQ

Anesthesia and Analgesia of Rats:

Parenteral Anesthesia:

Dissociatives

  • Ketamine, 50 mg/kg; IP; (sedative)
  • Katamine, 75-100 mg/kg + Xylazine 5-10mg/kg; IP
  • Ketamine, 22-44 mg/kg + Xylazine 2.5-10mg/kg + Acepromazine 0.75mg/kg; IP

Barbiturates

  • Pentobarbital, 35-50 mg/k; IP

Inhalant Anesthetic

  • Isoflurane 1-3% (up to 5% for induction)

Analgesics:

  • Ketoprofen, 2-5 mg/kg, q 12-24 hour; SQ
  • Buprenorphine, 0.01-0.05 mg/kg, q 6-12 hr.; SQ
  • Butorphanol, 1.0-2.0 mg/kg, q 4 hr; SQ
  • Carprofen 5 mg/kg, q 12-24 hr;SQ
  • Meloxicam 2 mg/kg, q 12-24 hr;SQ

 

Anesthesia and Analgesia of Fish and Amphibians

Immersion Anesthetics

  • Tricaine methane sulfonate (MS222), 300-500 mg/L (larva); 500-2000 mg/L (adults) (buffer with 10-25 m Eq/L sodium bicarbonate)
  • Benzocaine, 10-50 mg/L (larva); 300 mg/L (adults)

Injectable Anesthetics

  • Tricaine methane sulfonate, 100 mg/kg; SQ; IP; SQ
  • Tricaine methane sulfonate, 50-150 mg/kg; SQ; IM
  • Ketamine, 50-150 mg/kg; IM